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| Registros recuperados : 26 | |
| 2. |  | PEIXOTO, R. S.; CHAER, G. M.; FRANCO, N.; REIS JUNIOR, F. B. dos; MENDES, I. de C.; ROSADO, A. S. A decade of land use contributes to changes in the chemistry, biochemistry and bacterial community structures of soils in the Cerrado. Antonie van Leeuwenhoek, v. 98, n. 3, p. 403-413, 2010.| Biblioteca(s): Embrapa Agrobiologia; Embrapa Cerrados. |
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| 4. | | LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S. Comparison of DNA extraction protocols for microbial communities from soil treated with biochar. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 175-183, 2014.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Solos. |
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| 5. | | RACHID, C. T. C. de C.; BALIEIRO, F. de C.; PEIXOTO, R. S.; CHAER, G. M.; PICCOLO, M. de C.; ROSADO, A. S. Changes in soil microbial community and nitrogen cycling in mixed plantations of Eucalyptus urograndis and Acacia mangium culminates in soil nitrate increase. INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 14., 2012, Conpenhague, DE. The power of the small: abstracts... Wageningen, NE:The International Society for Microbial Ecology, 2012. ISME14| Biblioteca(s): Embrapa Agrobiologia. |
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| 6. | | LEITE, D. C. de A.; BALIEIRO, F. de C.; PIRES, C. de A.; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. da C.; PEIXOTO, R. S. Efeito de diferentes tipos de biomassa carbonizada (biocarvão) nas comunidades de bactérias e fungos associados às rizosferas de plantas de arroz (Oryza sativa) cultivar BRSMG curinga. In: CONGRESSO LATINOAMERICANO DE MICROBIOLOGIA, 21., 2012, Santos. Resumos [...]. São Paulo: Sociedade Brasileira de Microbiologia: Associação Latinoamericana de Microbiologia, 2012.| Biblioteca(s): Embrapa Solos. |
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| 7. | | RACHID, C. T. C. C.; SANTOS, A. L.; PICCOLO, M. C.; BALIEIRO, F. C.; COUTINHO, H. L. C.; PEIXOTO, R. S.; TIEDJE, J. M.; ROSADO, A. S. Effect of sugarcane burning or green harvest methods on the brazilian cerrado soil bacterial community structure. Plos One, v. 8, n. 3, e59342, Mar. 2013.| Biblioteca(s): Embrapa Solos. |
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| 8. | | TEIXEIRA, L. C. R. S.; YEARGEAU, E.; BALIEIRO, F. de C.; PICCOLO, M. C.; PEIXOTO, R. S.; GREER, C. W.; ROSADO, A. S. Plant and bird presence strongly influences the microbial communities in soils of admiralty bay, maritime antarctica. Plos One, v. 8, n. 6, jun. 2013.| Biblioteca(s): Embrapa Solos. |
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| 9. | | LEITE, D. C. de A.; BALIEIRO, F. de C.; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. da C.; PEIXOTO, R. S. Quantificação de genes-chave relacionados ao ciclo de nitrogênio microbiano em rizosfera plantas de arroz (Oryza sativa) cultivar BRSMG curinga tratadas com diferentes tipos de biocarvão. In: CONGRESSO LATINOAMERICANO DE MICROBIOLOGIA, 21., 2012, Santos. Resumos [...]. São Paulo: Sociedade Brasileira de Microbiologia: Associação Latinoamericana de Microbiologia, 2012.| Biblioteca(s): Embrapa Solos. |
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| 10. | | FONSECA, E. da S.; PEIXOTO, R. S.; ROSADO, A. S.; BALIEIRO, F. de C.; TIEDJE, J. M.; RACHID, C. T. C. da C. The microbiome of Eucalyptus roots under different management conditions and its potential for biological nitrogen fixation. Microbial Ecology, v. 75, n. 1, p. 183-191, 2018.| Biblioteca(s): Embrapa Solos. |
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| 11. | | RACHID, C. T. C. C.; BALIEIRO, F. de C.; PEIXOTO, R. S.; PINHEIRO, Y. A. S.; PICCOLO, M. C.; CHAER, G. M.; ROSADO, A. S. Mixed plantations can promote microbial integration and soil nitrate increases with changes in the N cycling genes. Soil Biology & Biochemistry, v. 66, p. 146-153, Nov. 2013.| Biblioteca(s): Embrapa Solos. |
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| 12. | | RACHID, C. T. C. C.; BALIEIRO, F. de C.; PEIXOTO, R. S.; PINHEIRO, Y, A. S.; PICOLLO, M. C.; CHAER, G. M. Mixed plantations can promote microbial integration and soil nitrate increases with changes in the N cycling genes. Soil Biology & Biochemistry, v. 66, p. 146-153, 2013.| Biblioteca(s): Embrapa Agrobiologia. |
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| 13. | | LEITE, D. C. A.; BALIEIRO, F. de C.; LEITE, L. F. C.; MADARI, B. E.; RIBEIRO, A. de F.; PEIXOTO, R. S.; ROSADO, A. S. Integrated crop-livestock-forest system presents a different soil microbial structure in comparison to other land uses. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Meio-Norte. |
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| 14. | | LEITE, D. C. A.; BALIEIRO, F. de C.; LEITE, L. F. C.; MADARI, B. E.; RIBEIRO, A. de F.; PEIXOTO, R. S.; ROSADO, A. S. Integrated crop-livestock-forest system presents a different soil microbial structure in comparison to other land uses. In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FOREST SYSTEMS; INTERNATIONAL SYMPOSIUM ON INTEGRATED CROP-LIVESTOCK SYSTEMS, 3., 2015, Brasília, DF. Towards sustainable intensification: proceedings. Brasília, DF: Embrapa, 2015.| Biblioteca(s): Embrapa Solos. |
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| 15. | | RACHID, C. T. C. C.; BALIEIRO, F. C.; FONSECA, E. S.; PEIXOTO, R. S.; CHAER, G. M.; TIEDJE, J. M.; ROSADO, A. S. Intercropped silviculture systems, a key to achieving soil fungal community management in eucalyptus plantations. Plos One, v. 10, n. 2, Feb. 2015.| Biblioteca(s): Embrapa Solos. |
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| 17. | | PEIXOTO, R. S.; COUTINHO, H. L. C.; MADARI, B.; MACHADO, P. L. O. A.; RUMJANEK, N. G.; VAN ELSAS, J. D.; SELDIN, L.; ROSADO, A. S. Soil aggregation and bacterial community structure as affected by tillage and cover cropping in the Brazilian cerrados. Soil & Tillage Research, Amsterdam, v. 90, n. 1-2, p. 16-28, nov. 2006.| Biblioteca(s): Embrapa Agrobiologia. |
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| 18. | | PIRES, C. A.; LEITE, D. C. A.; COSTA, C. T. R. C.; ANGELINI, G. A. R.; PEIXOTO, R. S.; ROSADO, A. S.; COUTINHO, H. L. C.; ZANATTA, J.; SALTON, J.; BALIEIRO, F. C. Impacto de diferentes níveis de palhada de cana-de-açúcar sobre comunidade fúngica total de latossolo do cerrado. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011.| Biblioteca(s): Embrapa Solos. |
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| 19. | | RACHID, C. T. C. da C.; LEITE, D. C. de A.; COUTINHO, H. L. da C.; BALIEIRO, F. de C.; PEIXOTO, R. S.; ROSADO, R. S.; PICCOLO, M. de C. Impacto da cana na comunidade de desnitrificantes, fluxo de N20 e potencial de desnitrificação do solo de cerrado. In: SIMPÓSIO CIENTÍFICO DOS PÓS-GRADUANDOS NO CENA/USP, 2., 2009, Piracicaba, SP. Anais... Piracicaba, SP: USP, 2009.| Biblioteca(s): Embrapa Solos. |
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| 20. | | RACHID, C. T. C. da C.; LEITE, D. C. de A.; CAVALINI, R. M.; PEIXOTO, R. S.; ROSADO, A. S.; BALIEIRO, F. de C.; PICCOLO, M. de C.; COUTINHO, H. L. da C. Estrutura da comunidade bacteriana e agregação do solo sob cerrado e cana-de-açúcar em Goiás. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas, PE. Anais... São Paulo: SBM, 2009.| Biblioteca(s): Embrapa Solos. |
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| Registros recuperados : 26 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Solos. |
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Data corrente: |
06/02/2015 |
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Data da última atualização: |
06/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S. |
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Afiliação: |
D. C. A. LEITE, UFRJ; FABIANO DE CARVALHO BALIEIRO, CNPS; C. A. PIRES; BEATA EMOKE MADARI, CNPAF; A. S. ROSADO, UFRJ; HEITOR LUIZ DA COSTA COUTINHO, CNPS; R. S. PEIXOTO, UFRJ. |
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Título: |
Comparison of DNA extraction protocols for microbial communities from soil treated with biochar. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 175-183, 2014. |
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DOI: |
10.1590/S1517-83822014000100023 |
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Idioma: |
Inglês |
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Conteúdo: |
Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples. MenosMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-... Mostrar Tudo |
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Palavras-Chave: |
Comunidades microbianas; Extração de DNA; Índice de pureza do DNA; PCR-DGGE. |
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Thesaurus NAL: |
biochar. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117433/1/BJMv45n1a23.pdf
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Marc: |
LEADER 02342naa a2200265 a 4500
001 2007986
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008 2014 bl uuuu u00u1 u #d
024 7 $a10.1590/S1517-83822014000100023$2DOI
100 1 $aLEITE, D. C. A.
245 $aComparison of DNA extraction protocols for microbial communities from soil treated with biochar.$h[electronic resource]
260 $c2014
520 $aMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples.
650 $abiochar
653 $aComunidades microbianas
653 $aExtração de DNA
653 $aÍndice de pureza do DNA
653 $aPCR-DGGE
700 1 $aBALIEIRO, F. C.
700 1 $aC. A. PIRES
700 1 $aMADARI, B. E.
700 1 $aROSADO, A. S.
700 1 $aCOUTINHO, H. L. C.
700 1 $aPEIXOTO, R. S.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 45, n. 1, p. 175-183, 2014.
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Embrapa Solos (CNPS) |
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